2381/36340 E. Torrado E. Torrado J. J. Fountain J. J. Fountain R. T. Robinson R. T. Robinson C. A. Martino C. A. Martino John Edward Pearl John Edward Pearl J. Rangel-Moreno J. Rangel-Moreno M. Tighe M. Tighe R. Dunn R. Dunn A. M. Cooper A. M. Cooper Differential and site specific impact of B cells in the protective immune response to Mycobacterium tuberculosis in the mouse University of Leicester 2016 Animals B-Lymphocytes Granulocyte-Macrophage Colony-Stimulating Factor Interleukin-10 Interleukin-6 Mice Mycobacterium tuberculosis Tuberculosis 2016-01-21 09:52:01 Journal contribution https://figshare.le.ac.uk/articles/journal_contribution/Differential_and_site_specific_impact_of_B_cells_in_the_protective_immune_response_to_Mycobacterium_tuberculosis_in_the_mouse/10134416 Cell-mediated immune responses are known to be critical for control of mycobacterial infections whereas the role of B cells and humoral immunity is unclear. B cells can modulate immune responses by secretion of immunoglobulin, production of cytokines and antigen-presentation. To define the impact of B cells in the absence of secreted immunoglobulin, we analyzed the progression of Mycobacterium tuberculosis (Mtb) infection in mice that have B cells but which lack secretory immunoglobulin (AID(-/-)µS(-/-)mice). AID(-/-)µS(-/-) mice accumulated a population of activated B cells in the lungs when infected and were more susceptible to aerosol Mtb when compared to wild type (C57BL/6) mice or indeed mice that totally lack B cells. The enhanced susceptibility of AID(-/-)µS(-/-) mice was not associated with defective T cell activation or expression of a type 1 immune response. While delivery of normal serum to AID(-/-)µS(-/-) mice did not reverse susceptibility, susceptibility in the spleen was dependent upon the presence of B cells and susceptibility in the lungs of AID(-/-)µS(-/-)mice was associated with elevated expression of the cytokines IL-6, GM-CSF, IL-10 and molecules made by alternatively activated macrophages. Blocking of IL-10 signaling resulted in reversal of susceptibility in the spleens and lungs of AID(-/-)µS(-/-) mice. These data support the hypothesis that B cells can modulate immunity to Mtb in an organ specific manner via the modulation of cytokine production and macrophage activation.