2381/11955648.v1
Yan Li
Yan
Li
Judith HI Haarhuis
Judith HI
Haarhuis
Angela Sedeno Cacciatore
Angela Sedeno
Cacciatore
Roel Oldenkamp
Roel
Oldenkamp
Marjon S van Ruiten
Marjon S
van Ruiten
Laureen Willems
Laureen
Willems
Hans Teunissen
Hans
Teunissen
Kyle W Muir
Kyle W
Muir
Elzo de Wit
Elzo
de Wit
Benjamin D Rowland
Benjamin D
Rowland
Daniel Panne
Daniel
Panne
The structural basis for cohesin-CTCF-anchored loops
University of Leicester
2020
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
FULLY-AUTOMATIC CHARACTERIZATION
HI-C REVEALS
DATA-COLLECTION
3D GENOME
WAPL
CHROMOSOMES
RESOLUTION
MECHANISM
TOPOLOGY
DOMAINS
2020-03-27 09:57:34
Journal contribution
https://figshare.le.ac.uk/articles/journal_contribution/The_structural_basis_for_cohesin-CTCF-anchored_loops/11955648
Cohesin catalyses the folding of the genome into loops that are anchored by CTCF1. The molecular mechanism of how cohesin and CTCF structure the 3D genome has remained unclear. Here we show that a segment within the CTCF N terminus interacts with the SA2–SCC1 subunits of human cohesin. We report a crystal structure of SA2–SCC1 in complex with CTCF at a resolution of 2.7 Å, which reveals the molecular basis of the interaction. We demonstrate that this interaction is specifically required for CTCF-anchored loops and contributes to the positioning of cohesin at CTCF binding sites. A similar motif is present in a number of established and newly identified cohesin ligands, including the cohesin release factor WAPL2,3. Our data suggest that CTCF enables the formation of chromatin loops by protecting cohesin against loop release. These results provide fundamental insights into the molecular mechanism that enables the dynamic regulation of chromatin folding by cohesin and CTCF.