Zamudio et al Environmental Microbiology 2020 SUPPELMENTARY DATA 10.1111/1462-2920.15111
Marco Rinaldo Oggioni
10.25392/leicester.data.12370145.v1
https://figshare.le.ac.uk/articles/dataset/Zamudio_et_al_Environmental_Microbiology_2020_SUPPELMENTARY_DATA_10_1111_1462-2920_15111/12370145
<p></p><p>Zamudio R,
RD Haigh, JD Ralph, M De Ste Croix, T Tasara, K Zurfluh, MJ Kwun, AD Millard,
SD Bentley, NJ Croucher, R Stephan, MR Oggioni. 2020. Lineage specific
evolution and gene flow in Listeria monocytogenes is independent of
bacteriophages. Environmental Microbiology. 2020 doi 10.1111/1462-2920.15111</p><p><br></p>
<p> </p>
<p>Listeria
monocytogenes is a foodborne pathogen causing systemic infection with high
mortality. To allow efficient tracing of outbreaks a clear definition of the
genomic signature of a cluster of related isolates is required, but lineage
specific characteristics call for a more detailed understanding of evolution.
In our work we used core genome MLST (cgMLST) to identify new outbreaks
combined to core genome SNP analysis to characterize the population structure
and gene flow between lineages. Whilst analysing differences between the four
lineages of L. monocytogenes we have detected differences in the recombination
rate, and interestingly also divergence in the SNP differences between
sub-lineages. In addition, the exchange of core genome variation between the
lineages exhibited a distinct pattern, with lineage III being the best donor
for horizontal gene transfer. Whilst attempting to link bacteriophage mediated
transduction to observed gene transfer, we found an inverse correlation between
phage presence in a lineage and the extent of recombination. Irrespective of
the profound differences in recombination rates observed between sub-lineages
and lineages we found that the previously proposed cut-off of 10 allelic
differences in cgMLST can be still considered valid for the definition of a
foodborne outbreak cluster of L. monocytogenes.</p><br><p></p>
2020-06-10 10:04:45
Supplementary materials Data