Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria.pdf (3.86 MB)
Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria.
journal contribution
posted on 2018-01-11, 09:47 authored by Rahul D. Kaduskar, Giulia Della Scala, Zaaima J. H. Al Jabri, Stefania Arioli, Loana Musso, Marco R. Oggioni, Sabrina Dallavalle, Diego MoraPromysalin was previously described as a narrow spectrum molecule with a unique species-specific activity against Pseudomonas aeruginosa. Here we demonstrate that promysalin is active against Gram-positive and Gram-negative bacteria using a microdilution assay. Promysalin acts on Gram-positive bacteria with a mechanism of action involving cell membrane damage with leakage of intracellular components. The evaluation of MICs and MBCs on 11 promysalin analogs, synthesized utilizing diverted total synthesis, allowed the identification of the structural moieties potentially involved in cell membrane interaction and damage. The mechanism of action of promysalin against Gram-negative bacteria is still not clarified, even if a synergistic effect with the bisguanidine chlorhexidine on cell membrane disruption has been observed.
Funding
We acknowledge Professor René De Mot, Centre of Microbial and Plant Genetics, Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium, for providing us P. putida RW10S1 and P. stutzeri LMG 2333.
History
Citation
Scientific Reports, 2017, 7:8861Author affiliation
/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Genetics and Genome BiologyVersion
- VoR (Version of Record)