University of Leicester
Browse
Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin..pdf (2.86 MB)

Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin.

Download (2.86 MB)
journal contribution
posted on 2015-10-08, 10:52 authored by A. Dupont, F. Mohamed, N. Salehen, S. Glenn, L. Francescut, R. Adib, S. Byrne, H. Brewin, I. Elliott, L. Richards, P. Dimitrova, W. Schwaeble, N. Ivanovska, A. Kadioglu, L. R. Machado, Peter W. Andrew, C. Stover
Streptococcus pneumoniae and Listeria monocytogenes, pathogens which can cause severe infectious disease in human, were used to infect properdin-deficient and wildtype mice. The aim was to deduce a role for properdin, positive regulator of the alternative pathway of complement activation, by comparing and contrasting the immune response of the two genotypes in vivo. We show that properdin-deficient and wildtype mice mounted antipneumococcal serotype-specific IgM antibodies, which were protective. Properdin-deficient mice, however, had increased survival in the model of streptococcal pneumonia and sepsis. Low activity of the classical pathway of complement and modulation of FcγR2b expression appear to be pathogenically involved. In listeriosis, however, properdin-deficient mice had reduced survival and a dendritic cell population that was impaired in maturation and activity. In vitro analyses of splenocytes and bone marrow-derived myeloid cells support the view that the opposing outcomes of properdin-deficient and wildtype mice in these two infection models is likely to be due to a skewing of macrophage activity to an M2 phenotype in the properdin-deficient mice. The phenotypes observed thus appear to reflect the extent to which M2- or M1-polarised macrophages are involved in the immune responses to S. pneumoniae and L. monocytogenes. We conclude that properdin controls the strength of immune responses by affecting humoral as well as cellular phenotypes during acute bacterial infection and ensuing inflammation.

History

Citation

Medical Microbiology and Immunology, 2014, 203 (4), pp. 257-271

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammation

Version

  • VoR (Version of Record)

Published in

Medical Microbiology and Immunology

Publisher

Springer Berlin Heidelberg

eissn

1432-1831

Copyright date

2014

Available date

2015-10-08

Publisher version

http://link.springer.com/article/10.1007/s00430-013-0324-z

Language

en

Usage metrics

    University of Leicester Publications

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC