University of Leicester
Browse
The Effects of Myo-inositol and Probiotics_FOR_SUBMISSION_after_review.pdf (400.02 kB)

The effects of myo-inositol and probiotic supplementation in a high-fat-fed preclinical model of glucose intolerance in pregnancy

Download (400.02 kB)
journal contribution
posted on 2020-03-12, 14:58 authored by JF Plows, JM Ramos Nieves, F Budin, K Mace, CM Reynolds, MH Vickers, I Silva-Zolezzi, PN Baker, JL Stanley
    Glucose intolerance during pregnancy - a major driver of gestational diabetes mellitus (GDM) - has significant short- and long-term health consequences for both the mother and child. As GDM prevalence continues to escalate, there is growing need for preventative strategies. There is limited but suggestive evidence that myo-inositol (MI) and probiotics (PB) could improve glucose tolerance during pregnancy. The present study tested the hypothesis that MI and/or PB supplementation would reduce the risk of glucose intolerance during pregnancy. Female C57BL/6 mice were randomised to receive either no treatment, MI, PB (Lactobacillus rhamnosus and Bifidobacterium lactis) or both (MIPB) for 5 weeks. They were then provided with a high-fat diet for 1 week before mating commenced and throughout mating/gestation, while remaining on their respective treatments. An oral glucose tolerance test occurred at gestational day (GD) 16·5 and tissue collection at GD 18·5. Neither MI nor PB, separately or combined, improved glucose tolerance. However, MI and PB both independently increased adipose tissue expression of Ir, Irs1, Akt2 and Pck1, and PB also increased Pparγ. MI was associated with reduced gestational weight gain, whilst PB was associated with increased maternal fasting glucose, total cholesterol and pancreas weight. These results suggest that MI and PB may improve insulin intracellular signalling in adipose tissue but this did not translate to meaningful differences in glucose tolerance. The absence of fasting hyperglycaemia or insulin resistance suggests this is a very mild model of GDM, which may have affected our ability to assess the impact of these nutrients.

Funding

This work was funded by Nestec Ltd. The funders contributed to the design of the study and approved the final version of the manuscript, but did not conduct the study, analyse the data or write the manuscript. The maltodextrin used for control purposes was donated by Axieo Specialties.

History

Citation

British Journal of Nutrition (2020), Volume 123, Issue 5, 516–528

Author affiliation

College of Life Sciences

Version

  • AM (Accepted Manuscript)

Published in

The British Journal of Nutrition

Volume

123

Issue

5

Pagination

516 - 528

Publisher

Cambridge University Press (CUP)

issn

0007-1145

eissn

1475-2662

Acceptance date

2019-10-29

Copyright date

2019

Publisher DOI

Publisher version

https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/effects-of-myoinositol-and-probiotic-supplementation-in-a-highfatfed-preclinical-model-of-glucose-intolerance-in-pregnancy/9A4A594BAF96EC6805E5BA45E90C19C5

Spatial coverage

England

Language

eng

Usage metrics

    University of Leicester Publications

    Categories

    Keywords

    DiabetesGestational diabetesGlucose intoleranceHigh-fat dietInositolMyo-inositolProbioticsControl dietGestational dayGestational diabetes mellitusHFD with myo-inositol added to the dietHFMI and probiotic added to drinking waterHFD with probiotic added to drinking waterhomeostatic model assessment of insulin resistance

    Licence

    All Rights Reserved

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC