Appendix I: PCR Design

This appendix includes PCR designs for the recombination hotspot crossover assays done in the DNA3 and newly described AA hotspots in the MHCII, duplex PCR for screening for K-ZnF containing PRDM9 alleles in a British ALL cohort and Sanger sequencing as carried out in this thesis. PCR primer characteristics, PCR cycling conditions, PCR strategy for target regions and sequence context of target regions, AA hotspot morphology analysis are included.