srep37638.pdf (753.66 kB)
Download file

A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format.

Download (753.66 kB)
journal contribution
posted on 29.11.2016, 10:44 by Katarzyna Smolinska-Kempisty, Antonio Guerreiro, Francesco Canfarotta, César Cáceres, Michael J. Whitcombe, Sergey Piletsky
Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked competitive assays. Imprinted nanoparticles-based assays showed detection limits in the pM range and polymer-coated microplates are stable to storage at room temperature for at least 1 month. No response to analyte was detected in control experiments with nanoparticles imprinted with an unrelated template (trypsin) but prepared with the same polymer composition. The ease of preparation, high affinity of solid-phase synthesised imprinted nanoparticles and the lack of requirement for cold chain logistics make them an attractive alternative to traditional antibodies for use in immunoassays.

Funding

The authors thank the University of Leicester for financial support through the Proof of Concept Fund.

History

Citation

Scientific Reports, 2016, 6:37638

Author affiliation

/Organisation/COLLEGE OF SCIENCE AND ENGINEERING/Department of Chemistry

Version

VoR (Version of Record)

Published in

Scientific Reports

Publisher

Nature Publishing Group

issn

2045-2322

eissn

2045-2322

Acceptance date

28/10/2016

Available date

29/11/2016

Publisher version

http://www.nature.com/articles/srep37638

Language

en

Usage metrics

Categories

Licence

Exports