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A pulsed current electric field alters protein expression creating a wound healing phenotype in human skin cells

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journal contribution
posted on 27.08.2020, 11:00 by LE Bostan, S Almqvist, Christine E Pullar
Aim: Pulsed current (PC) electric field (EF) devices promote healing in chronic wounds but the underpinning mechanisms are largely unknown. The gap between clinical evidence and mechanistic understanding limits device uptake in clinics. Materials & methods: Migration, proliferation and gene/protein expression profiles were investigated in the presence/absence of PCEF, in skin: keratinocytes (NHK); dermal fibroblasts (HDF); dermal microvascular endothelial cells (HDMEC) and macrophages (THP-1). Results: While PCEF had little effect on migration or proliferation, it significantly altered the expression of 31 genes and the secretion of 7 pro-angiogenic and pro-regenerative growth factors using ELISAs. Conclusion: PCEF significantly altered skin cell genomes/proteomes which provides some evidence of how PCEF devices promote healing of chronic wounds.

History

Citation

Regenerative medicine, 15 (5), 2020

Author affiliation

Department of Molecular & Cell Biology

Version

AM (Accepted Manuscript)

Published in

Regenerative medicine

Volume

15

Issue

5

Pagination

1611 - 1623

Publisher

Future Medicine Ltd

issn

1746-0751

eissn

1746-076X

Copyright date

2020

Available date

07/07/2021

Spatial coverage

England

Language

eng

Publisher version

https://www.futuremedicine.com/doi/abs/10.2217/rme-2019-0087