A_spontaneous_mutation_in_MutL_Homolog_3_HvMLH3_affects_synapsis_and_crossover_resolution_in_the_barley_desynaptic_mutant_des10_Colas_2016.pdf (322.92 kB)
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A spontaneous mutation in MutL-Homolog 3 (HvMLH3) affects synapsis and crossover resolution in the barley desynaptic mutant des10

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posted on 01.02.2019, 09:31 by I Colas, M Macaulay, JD Higgins, D Phillips, A Barakate, M Posch, SJ Armstrong, FC Franklin, C Halpin, R Waugh, L Ramsay
Although meiosis is evolutionarily conserved, many of the underlying mechanisms show species-specific differences. These are poorly understood in large genome plant species such as barley (Hordeum vulgare) where meiotic recombination is very heavily skewed to the ends of chromosomes. The characterization of mutant lines can help elucidate how recombination is controlled. We used a combination of genetic segregation analysis, cytogenetics, immunocytology and 3D imaging to genetically map and characterize the barley meiotic mutant DESYNAPTIC 10 (des10). We identified a spontaneous exonic deletion in the orthologue of MutL-Homolog 3(HvMlh3) as the causal lesion. Compared with wild-type,des10 mutants exhibit reduced recombination and fewer chiasmata, resulting in the loss of obligate crossovers and leading tochromosome mis-segregation. Using 3D structured illumination microscopy (3D-SIM), we observed that normal synapsis progression was also disrupted in des10, a phenotype that was not evident with standard confocal microscopy and that has not been reported with Mlh3 knockout mutants in Arabidopsis. Our data provide new insights on the interplay between synapsis and recombination in barley and highlight the need for detailed studies of meiosis in non model species. This study also confirms the importance of early stages of prophase I for the control of recombination in large genome cereals.

Funding

The research leading to these results has received funding from the European Community’s Seventh Framework Programme FP7/2007-2013 under grant agreement no. 222883 to R.W., S.J.A. and F.C.H.F.

History

Citation

New Phytologist (2016) 212:693–707

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Genetics and Genome Biology

Version

AO (Author's Original)

Published in

New Phytologist (2016) 212:693–707

Publisher

Wiley for New Phytologist Trust

eissn

1469-8137

Acceptance date

11/05/2016

Copyright date

2016

Available date

01/02/2019

Publisher version

https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.14061

Language

en

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