Aggregation-Prone Proteins Modulate Huntingtin Inclusion Body Formation in Yeast – PLOS Currents Huntington Disease.pdf (402.59 kB)
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Aggregation-prone proteins modulate huntingtin inclusion body formation in yeast

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posted on 22.02.2016, 11:54 by Ralitsa B. Kantcheva, Robert Mason, Flaviano Giorgini
Huntington's disease (HD) is a fatal neurodegenerative disorder caused by a polyglutamine expansion in the huntingtin (HTT) protein. The expression of mutant HTT in the baker's yeast Saccharomyces cerevisiae recapitulates many of the cellular phenotypes observed in mammalian HD models. Mutant HTT aggregation and toxicity in yeast is influenced by the presence of the Rnq1p and Sup35p prions, as well as other glutamine/asparagine-rich aggregation-prone proteins. Here we investigated the ability of a subset of these proteins to modulate mutant HTT aggregation and to substitute for the prion form of Rnq1p. We find that overexpression of either the putative prion Ybr016wp or the Sup35p prion restores aggregation of mutant HTT in yeast cells lacking the Rnq1p prion. These results indicate that an interchangeable suite of aggregation-prone proteins regulates mutant HTT aggregation dynamics in yeast, which may have implications for mutant HTT aggregation in human cells.

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Citation

PLoS Currents , 2014, 6(1)

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Department of Genetics

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VoR (Version of Record)

Published in

PLoS Currents

Publisher

Public Library of Science

eissn

2157-3999

Copyright date

2014

Available date

22/02/2016

Publisher version

http://currents.plos.org/hd/article/aggregation-prone-proteins-modulate-huntingtin-inclusion-body-formation-in-yeast/

Language

en

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