Allele specific expression and methylation in the bumblebee, Bombus terrestris.pdf (544.34 kB)
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Allele specific expression and methylation in the bumblebee, Bombus terrestris

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posted on 10.04.2018, 14:13 by Zoe Lonsdale, Kate Lee, Maria Kiriakidu, Harindra Amarasinghe, Despina Nathanael, Catherine J. O'Connor, Eamonn B. Mallon
The social hymenoptera are emerging as models for epigenetics. DNA methylation, the addition of a methyl group, is a common epigenetic marker. In mammals and flowering plants methylation affects allele specific expression. There is contradictory evidence for the role of methylation on allele specific expression in social insects. The aim of this paper is to investigate allele specific expression and monoallelic methylation in the bumblebee, Bombus terrestris. We found nineteen genes that were both monoallelically methylated and monoallelically expressed in a single bee. Fourteen of these genes express the hypermethylated allele, while the other five express the hypomethylated allele. We also searched for allele specific expression in twenty-nine published RNA-seq libraries. We found 555 loci with allele-specific expression. We discuss our results with reference to the functional role of methylation in gene expression in insects and in the as yet unquantified role of genetic cis effects in insect allele specific methylation and expression.

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Citation

PeerJ, 2017, 5:e3798

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Genetics and Genome Biology

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VoR (Version of Record)

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PeerJ

Publisher

PeerJ

eissn

2167-8359

Acceptance date

21/08/2017

Copyright date

2017

Available date

10/04/2018

Publisher version

https://peerj.com/articles/3798/

Language

en

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