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Application of a mitochondrial DNA control region frequency database for UK domestic cats

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journal contribution
posted on 20.12.2016, 16:44 by Barbara Ottolini, Gurdeep Matharu Lall, Federico Sacchini, Mark A. Jobling, Jon H. Wetton
DNA variation in 402 bp of the mitochondrial control region flanked by repeat sequences RS2 and RS3 was evaluated by Sanger sequencing in 152 English domestic cats, in order to determine the significance of matching DNA sequences between hairs found with a victim’s body and the suspect’s pet cat. Whilst 95% of English cats possessed one of the twelve globally widespread mitotypes, four new variants were observed, the most common of which (2% frequency) was shared with the evidential samples. No significant difference in mitotype frequency was seen between 32 individuals from the locality of the crime and 120 additional cats from the rest of England, suggesting a lack of local population structure. However, significant differences were observed in comparison with frequencies in other countries, including the closely neighbouring Netherlands, highlighting the importance of appropriate genetic databases when determining the evidential significance of mitochondrial DNA evidence.

History

Citation

Forensic Science International: Genetics, 2017, 27, pp. 149–155

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Cancer Studies and Molecular Medicine

Version

AM (Accepted Manuscript)

Published in

Forensic Science International: Genetics

Publisher

Elsevier

issn

1872-4973

Acceptance date

20/12/2016

Copyright date

2016

Available date

21/12/2017

Publisher version

http://www.sciencedirect.com/science/article/pii/S1872497316302411

Notes

The file associated with this record is under a 12 month embargo from publication in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.

Language

en