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Bidirectional promoters in seed development and related hormone/stress responses

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journal contribution
posted on 10.07.2015, 08:27 by Sofia Kourmpetli, Kate Lee, R. Hemsley, P. Rossignol, Thaleia Papageorgiou, Sinéad Drea
Background: Bidirectional promoters are common in genomes but under-studied experimentally, particularly in plants. We describe a targeted identification and selection of a subset of putative bidirectional promoters to identify genes involved in seed development and to investigate possible coordinated responses of gene pairs to conditions important in seed maturation such as desiccation and ABA-regulation. Results: We combined a search for 100–600 bp intergenic regions in the Arabidopsis genome with a cis-element based selection for those containing multiple copies of the G-box motif, CACGTG. One of the putative bidirectional promoters identified also contained a CE3 coupling element 5 bp downstream of one G-box and is identical to that characterized previously in the HVA1 promoter of barley. CE3 elements are significantly under-represented and under-studied in Arabidopsis. We further characterized the pair of genes associated with this promoter and uncovered roles for two small, previously uncharacterized, plant-specific proteins in Arabidopsis seed development and stress responses. Conclusions: Using bioinformatics we identified putative bidirectional promoters involved in seed development and analysed expression patterns for a pair of plant-specific genes in various tissues and in response to hormones/stress. We also present preliminary functional analysis of these genes that is suggestive of roles in seed development.

History

Citation

BMC Plant Biology, 2013, 13 : 187

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Biology

Version

VoR (Version of Record)

Published in

BMC Plant Biology

Publisher

BioMed Central

issn

1471-2229

Acceptance date

15/11/2013

Copyright date

2013

Available date

10/07/2015

Publisher version

http://www.biomedcentral.com/1471-2229/13/187

Language

en