Connexin40 regulates platelet function..pdf (1.05 MB)
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Connexin40 regulates platelet function

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journal contribution
posted on 14.10.2015, 08:21 by S. Vaiyapuri, L. A. Moraes, T. Sage, M. S. Ali, K. R. Lewis, Martyn P. Mahaut-Smith, E. Oviedo-Orta, A. M. Simon, J. M. Gibbins
The presence of multiple connexins was recently demonstrated in platelets, with notable expression of Cx37. Studies with Cx37-deficient mice and connexin inhibitors established roles for hemichannels and gap junctions in platelet function. It was uncertain, however, whether Cx37 functions alone or in collaboration with other family members through heteromeric interactions in regulation of platelet function. Here we report the presence and functions of an additional platelet connexin, Cx40. Inhibition of Cx40 in human platelets or its deletion in mice reduces platelet aggregation, fibrinogen binding, granule secretion and clot retraction. The effects of the Cx37 inhibitor [superscript: 37,43]Gap27 on Cx40[superscript: -/-] mouse platelets and of the Cx40 inhibitor [superscript: 40]Gap27 on Cx37[superscript: -/-] mouse platelets revealed that each connexin is able to function independently. Inhibition or deletion of Cx40 reduces haemostatic responses in mice, indicating the physiological importance of this protein in platelets. We conclude that multiple connexins are involved in regulating platelet function, thereby contributing to haemostasis and thrombosis.

History

Citation

Nature Communications, 2013, 4, 2564

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Molecular & Cell Biology

Version

VoR (Version of Record)

Published in

Nature Communications

Publisher

Nature Publishing Group

eissn

2041-1723

Acceptance date

06/09/2013

Copyright date

2013

Available date

14/10/2015

Publisher version

http://www.nature.com/ncomms/2013/131007/ncomms3564/full/ncomms3564.html#affil-auth

Language

en