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Conservation, divergence and functions of centromeric satellite DNA families in the Bovidae.

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journal contribution
posted on 01.05.2019, 09:08 by A Escudeiro, F Adega, TJ Robinson, JS Heslop-Harrison, R Chaves
Repetitive satellite DNA (satDNA) sequences are abundant in eukaryote genomes, with a structural and functional role in centromeric function. We analysed the nucleotide sequence and chromosomal location of the five known cattle (Bos taurus) satDNA families in seven species from the tribe Tragelaphini (Bovinae subfamily). One of the families (SAT1.723) was present at the chromosomes' centromeres of the Tragelaphini species, as well in two more distantly related bovid species, Ovis aries and Capra hircus. Analysis of the interaction of SAT1.723 with centromeric proteins revealed that this satDNA sequence is involved in the centromeric activity in all the species analysed and that it is preserved for at least 15-20 My across Bovidae species. The satDNA sequence similarity among the analysed species reflected different stages of homogeneity/heterogeneity, revealing the evolutionary history of each satDNA family. The SAT1.723 monomer-flanking regions showed the presence of transposable elements, explaining the extensive shuffling of this satDNA between different genomic regions.

Funding

This work was supported by PhD grant SFRH/BD/98122/2013 from the Science and Technology Foundation (FCT) from Portugal. This work was funded by the BioISI project UID/Multi/04046/2019.

History

Citation

Genome Biology and Evolution, 2019, 11(4), pp. 1152–1165

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Genetics and Genome Biology

Version

VoR (Version of Record)

Published in

Genome Biology and Evolution

Publisher

Oxford University Press (OUP) for Society for Molecular Biology and Evolution

eissn

1759-6653

Acceptance date

11/03/2019

Copyright date

2019

Available date

01/05/2019

Publisher version

https://academic.oup.com/gbe/article/11/4/1152/5393269

Notes

Supplementary data are available at Genome Biology and Evolution online. https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/gbe/11/4/10.1093_gbe_evz061/1/evz061_supp.docx?Expires=1556795138&Signature=BREYg2PqmgYTsCpxSLTyLQdkHyUUgr-ZPwXCJaerioHlkqX8XnLuVZtdTywh2LmObX6BKtFQqz7P5sFRjEUWkuZI9AzSwVLGvKkxa6mGfew-QO2WPLZl7eujjFdWI-Nxok7zzZ4UOSz1bf2fE4ycU-8yYLhP4Ftq3fA1KF6QhSdMyydNddJZmfVbcXMV8ZwnsAs1zUVX10v~hf120ThU8CmoykRMtFyHXa~NZDoxWopq8ZKUMiUjJdqM6dqkGr2XsTOReXU1YaLeqT1bdieD-akcbY51ALIo2JSxLkjIWmOxTGJOrVisWxMJOpKNJKcqPq8oNnzcsPJSpAq96E0OtA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA This project data have been deposited at GenBank (NCBI) under the acession numbers MK499473 to MK499615. http://www.ncbi.nlm.nih.gov/nuccore/MK499473 http://www.ncbi.nlm.nih.gov/nuccore/MK499615

Language

en