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Cytological techniques to analyse meiosis in Arabidopsis arenosa for investigating adaptation to polyploidy

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journal contribution
posted on 20.03.2014, 10:28 by James D. Higgins, Kevin M. Wright, Kirsten Bomblies, F. Chris H. Franklin
Arabidopsis arenosa is a close relative of the model plant A. thaliana, and exists in nature as stable diploid and autotetraploid populations. Natural tetraploids have adapted to whole genome duplication and do not commonly show meiotic errors such as multivalent and univalent formation, which can lead to chromosome non-disjunction and reduced fertility. A genome scan for genes strongly differentiated between diploid and autotetraploid A. arenosa identified a subset of meiotic genes that may be responsible for adaptation to polyploid meiosis. To investigate the mechanisms by which A. arenosa adapted to its polyploid state, and the functionality of the identified potentially adaptive polymorphisms, a thorough cytological analysis is required. Therefore, in this chapter we describe methods and techniques to analyze male meiosis in A. arenosa, including optimum plant growth conditions, and immunocytological and cytological approaches developed with the specific purpose of understanding meiotic adaptation in an autotetraploid. In addition we present a meiotic cytological atlas to be used as a reference for particular stages and discuss observations arising from a comparison of meiosis between diploid and autotetraploid A. arenosa.

Funding

The F. Chris H. Franklin laboratory is supported by the BBSRC. Kevin M. Wright is supported by NIH fellowship # 1F32GM105293-01 and a Harvard Postdoctoral Award for Professional Development.

History

Citation

Frontiers in Plant Science, 2014, 4:546

Version

VoR (Version of Record)

Published in

Frontiers in Plant Science

Publisher

Frontiers

issn

1664-462X

eissn

1664-462X

Copyright date

2014

Available date

20/03/2014

Publisher version

http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00546/full

Language

en