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Detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic DNA probe

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journal contribution
posted on 13.12.2016, 16:04 by S. L. Thein1, D. G. Oscier, A. J. Jeffreys, C. Hesketh, S. Pilkington, C. Summers, M. Fitchett, J. S . Wainscoat
Chromosomal loss is a characteristic feature of the myelodysplastic syndromes (MDS). A method is described which detects chromosomal 7 loss in MDS by DNA analysis using a specific hypervariable region gene probe which has been cloned from a human DNA fingerprint. Loss of one of the chromosomal 7 homologues was demonstrated in 10/118 MDS patients; the ten patients include all the five patients which had previously been shown to have monosomy 7 by cytogenetic analysis. This technique makes it feasible to study serial samples from large numbers of patients for loss of chromosomal material and could be readily applied to the study of other human malignancies.

Funding

SLT is a Wellcome Senior Research Fellow in Clinical Science. JSW is supported in part by the Leukaemia Research Fund.

History

Citation

British Journal of Cancer (1988) 57, 131–134

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Department of Genetics

Version

VoR (Version of Record)

Published in

British Journal of Cancer (1988) 57

Publisher

Cancer Research UK, Nature Publishing Group

issn

0007-0920

eissn

1532-1827

Available date

13/12/2016

Publisher version

http://www.nature.com/bjc/journal/v57/n2/abs/bjc198827a.html

Language

en

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