Electrochemical Detection of Plasma Immunoglobulin as a Biomarker for Alzheimer's Disease.pdf (1.49 MB)
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Electrochemical Detection of Plasma Immunoglobulin as a Biomarker for Alzheimer's Disease

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journal contribution
posted on 14.06.2018, 15:13 by Goulielmos-Zois Garyfallou, Orlando Ketebu, Samet Şahin, Elizabeth B. Mukaetova-Ladinska, Michael Catt, Eileen Hao Yu
The clinical diagnosis and treatment of Alzheimer's disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 10-9 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL-1 range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices.

History

Citation

Sensors, 2017, 17 (11), 2464

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Neuroscience, Psychology and Behaviour

Version

VoR (Version of Record)

Published in

Sensors

Publisher

MDPI

eissn

1424-8220

Acceptance date

20/10/2017

Copyright date

2017

Available date

14/06/2018

Publisher version

http://www.mdpi.com/1424-8220/17/11/2464

Language

en