Genetic interactions between ABA signalling and the Arg?N-end rule pathway during Arabidopsis seedling establishment.pdf (2.3 MB)
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Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment.

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posted on 17.09.2019, 15:21 by H Zhang, L Gannon, PD Jones, CA Rundle, KL Hassall, DJ Gibbs, MJ Holdsworth, FL Theodoulou
The Arg/N-end rule pathway of ubiquitin-mediated proteolysis has multiple functions throughout plant development, notably in the transition from dormant seed to photoautotrophic seedling. PROTEOLYSIS6 (PRT6), an N-recognin E3 ligase of the Arg/N-end rule regulates the degradation of transcription factor substrates belonging to Group VII of the Ethylene Response Factor superfamily (ERFVIIs). It is not known whether ERFVIIs are associated with all known functions of the Arg/N-end rule, and the downstream pathways influenced by ERFVIIs are not fully defined. Here, we examined the relationship between PRT6 function, ERFVIIs and ABA signalling in Arabidopsis seedling establishment. Physiological analysis of seedlings revealed that N-end rule-regulated stabilisation of three of the five ERFVIIs, RAP2.12, RAP2.2 and RAP2.3, controls sugar sensitivity of seedling establishment and oil body breakdown following germination. ABA signalling components ABA INSENSITIVE (ABI)4 as well as ABI3 and ABI5 were found to enhance ABA sensitivity of germination and sugar sensitivity of establishment in a background containing stabilised ERFVIIs. However, N-end rule regulation of oil bodies was not dependent on canonical ABA signalling. We propose that the N-end rule serves to control multiple aspects of the seed to seedling transition by regulation of ERFVII activity, involving both ABA-dependent and independent signalling pathways.

Funding

We thank Ruth Finkelstein for abi4–1 seeds, Eiji Nambara for Col-0 × abi3–6 seeds, and Cristina Sousa-Correia for maintenance of prt6-1+/− snrk2.2 2.3 2.6 lines. We are grateful to Smita Kurup and Kirsty Halsey for assistance with confocal microscopy and Graham Shephard for photography. Research at Rothamsted was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) through grant BB/J016276/1 and the Tailoring Plant Metabolism (TPM) Institute Strategic Grant BBS/E/C/000I0420. Research at Nottingham was funded by a BBSRC PhD studentship to PDJ.

History

Citation

Scientific Reports, 2018, volume 8, Article number: 15192

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Department of Cardiovascular Sciences

Version

VoR (Version of Record)

Published in

Scientific Reports

Publisher

Nature Research (part of Springer Nature)

eissn

2045-2322

Acceptance date

01/10/2018

Copyright date

2018

Available date

17/09/2019

Publisher version

https://www.nature.com/articles/s41598-018-33630-5

Notes

All data generated or analysed during this study are included in this published article (and its Supplementary Information files). Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-018-33630-5.

Language

en

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