rccm.201905-1017oc.pdf (925.4 kB)
Download file

Genome-Wide Association Study of Susceptibility to Idiopathic Pulmonary Fibrosis

Download (925.4 kB)
journal contribution
posted on 29.04.2020, 14:38 by Richard Allen, Beatriz Guillen-Guio, Justin M Oldham, Shwu-Fan Ma, Amy Dressen, megan paynton, luke kraven, Ma'en Obeida, Xuan Li, Michael Ng, Rebecca Braybrooke, Maria Molina-Molina, Brian D Hobbs, Rachel K Putman, Phuwanat Sakornsakolpat, Helen L Booth, William A Fahy, Simon P Hart, Mike R Hill, Nik Hirani, Richard B Hubbard, Robin J McAnulty, Ann B Millar, Vidyia Navaratnam, Eunice OballaEunice Oballa, Helen Parfrey, Gauri Saini, Yingze Zhang, Naftali Kaminski, Ayodeji Adegunsoye, Mary E Strek, Margaret Neighbors, Xuting R Sheng, Gunnar Gudmundsson, Vilmundur Gudnason, Hiroto Hatabu, David J Lederer, Ani Manichaikul, John D Newell Jr, George T O'Connor, Victor E Ortega, Hanfei Xu, Tasha E Fingerlin, Yohan Bossé, Ke Hao, Philippe Joubert, David C Nickle, Don D Sin, Wim Timens, Dominic Furniss, Andrew P Morris, Krina Zondervan, Ian P Hall, Ian Sayers, Martin D Tobin, Toby M Maher, Michael H Cho, Gary M Hunninghake, David A Schwartz, Brian L Yaspan, Philip L Molyneaux, Carlos Flores, Imre Noth, R Gisli Jenkins, Louise V Wain
Rationale: Idiopathic pulmonary fibrosis (IPF) is a complex lung disease characterised by scarring of the lung that is believed to result from an atypical response to injury of the epithelium. Genome-wide association studies have reported signals of associationimplicating multiple pathways including host defence, telomere maintenance, signalling and cell-cell adhesion.
Objectives: To improve our understanding of factors that increase IPF susceptibility by identifying previously unreported genetic associations.
Methods and measurements: We conducted genome-wide analyses across three independent studies and meta-analysed these results to generate the largest genome-wide association study of IPF to date (2,668 IPF cases and 8,591 controls). We performed replication in two independent studies (1,456 IPF cases and 11,874 controls) and functionalanalyses (including statistical fine-mapping, investigations into gene expression and testing for enrichment of IPF susceptibility signals in regulatory regions) to determine putatively causal genes. Polygenic risk scores were used to assess the collective effect of variants not reported as associated with IPF.
Main results: We identified and replicated three new genome-wide significant (P<5×10−8) signals of association with IPF susceptibility (associated with altered gene expression of KIF15, MAD1L1 and DEPTOR) and confirmed associations at 11 previously reported loci. Polygenic risk score analyses showed that the combined effect of many thousands of as-yet unreported IPF susceptibility variants contribute to IPF susceptibility.
Conclusions: The observation that decreased DEPTOR expression associates with increased susceptibility to IPF, supports recent studies demonstrating the importance of mTOR signalling in lung fibrosis. New signals of association implicating KIF15 and MAD1L1 suggest a possible role of mitotic spindle-assembly genes in IPF susceptibility.

History

Citation

American Journal of Respiratory and Critical Care Medicine, in press.

Author affiliation

Department of Health Sciences, University of Leicester

Version

VoR (Version of Record)

Published in

American Journal of Respiratory and Critical Care Medicine

Volume

201

Issue

5

Publisher

American Thoracic Society

issn

1073-449X

eissn

1535-4970

Acceptance date

07/11/2019

Copyright date

2019

Available date

11/11/2019

Language

en

Usage metrics

Categories

Licence

Exports