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Genomic and proteomic characterization of two novel siphovirus infecting the sedentary facultative epibiont cyanobacterium Acaryochloris marina

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posted on 12.09.2016, 14:40 by Yi-Wah Chan, Andrew D. Millard, Peter J. Wheatley, Antony B. Holmes, Remus Mohr, Anna L. Whitworth, Nicholas H. Mann, Anthony W. D. Larkum, Wolfgang R. Hess, David J. Scanlan, Martha R. J. Clokie
Summary: Acaryochloris marina is a symbiotic species of cyanobacteria that is capable of utilizing far-red light. We report the characterization of the phages A-HIS1 and A-HIS2, capable of infecting Acaryochloris. Morphological characterization of these phages places them in the family Siphoviridae. However, molecular characterization reveals that they do not show genetic similarity with any known siphoviruses. While the phages do show synteny between each other, the nucleotide identity between the phages is low at 45–67%, suggesting they diverged from each other some time ago. The greatest number of genes shared with another phage (a myovirus infecting marine Synechococcus) was four. Unlike most other cyanophages and in common with the Siphoviridae infecting Synechococcus, no photosynthesis-related genes were found in the genome. CRISPR (clustered regularly interspaced short palindromic repeats) spacers from the host Acaryochloris had partial matches to sequences found within the phages, which is the first time CRISPRs have been reported in a cyanobacterial/cyanophage system. The phages also encode a homologue of the proteobacterial RNase T. The potential function of RNase T in the mark-up or digestion of crRNA hints at a novel mechanism for evading the host CRISPR system.

History

Citation

Environmental Microbiology, 2015, 17 (11), pp. 4239-4252

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammation

Version

VoR (Version of Record)

Published in

Environmental Microbiology

Publisher

Wiley on behalf of Society for Applied Microbiology (SfAM)

issn

1462-2912

eissn

1462-2920

Copyright date

2015

Available date

12/09/2016

Publisher version

http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12735/abstract

Language

en