Ha-ras restriction fragment length polymorphisms in colorectal cancer..pdf (1.24 MB)
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Ha-ras restriction fragment length polymorphisms in colorectal cancer.

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journal contribution
posted on 13.12.2016, 15:54 by F. S. Wyllie, V. Wynford-Thomas, N. R. Lemoine, G. T. Williams, E. D. Williams, D. Wynford-Thomas
The possibility of an association between restriction fragment length polymorphisms (RFLPs) at the Ha-ras gene locus and susceptibility to develop colorectal cancer has been investigated. Leucocyte DNA from 46 carcinoma patients and 49 controls was analysed by Southern blotting to determine the size distribution of restriction fragments containing the variable tandem repeat 3' to the Ha-ras gene. Four predominant allelic fragments were found in both groups (in AvaII digests having sizes of 1.55, 2.0, 2.65 and 3.15 kilobases [kb]), together with a variety of 'rare' alleles (with individual frequencies less than 5%). The overall prevalence of rare alleles was not significantly different between cancer and control groups. The distribution of the common alleles, however, differed significantly. The combined frequency of the two larger alleles (a3 and a4) was approximately twice as high in the cancer group (34%) as in controls (18%) (P less than 0.025), which was reflected in a highly significant increase in the proportion of individuals carrying an a3 or a4 allele.

Funding

We are grateful to the Cancer Research Campaign for grant support, and to Mr B.I. Rees and Mr H.L. Young for access to clinical samples.

History

Citation

British Journal of Cancer, 1988, 57 (2), pp. 135-138

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine

Version

VoR (Version of Record)

Published in

British Journal of Cancer

Publisher

Cancer Research UK, Nature Publishing Group

issn

0007-0920;1532-1827

Available date

13/12/2016

Publisher version

http://www.nature.com/bjc/journal/v57/n2/abs/bjc198828a.html

Language

en