Invasiveness and metastasis of retinoblastoma in an orthotopic zebrafish tumor model..pdf (1.63 MB)
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Invasiveness and metastasis of retinoblastoma in an orthotopic zebrafish tumor model.

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posted on 27.01.2016, 11:13 by X. Chen, J. Wang, Z. Cao, K. Hosaka, L. Jensen, H. Yang, Y. Sun, R. Zhuang, Y. Liu, Yihai Cao
Retinoblastoma is a highly invasive malignant tumor that often invades the brain and metastasizes to distal organs through the blood stream. Invasiveness and metastasis of retinoblastoma can occur at the early stage of tumor development. However, an optimal preclinical model to study retinoblastoma invasiveness and metastasis in relation to drug treatment has not been developed. Here, we developed an orthotopic zebrafish model in which retinoblastoma invasion and metastasis can be monitored at a single cell level. We took the advantages of immune privilege and transparent nature of developing zebrafish embryos. Intravitreal implantation of color-coded retinoblastoma cells allowed us to kinetically monitor tumor cell invasion and metastasis. Further, interactions between retinoblastoma cells and surrounding microvasculatures were studied using a transgenic zebrafish that exhibited green fluorescent signals in blood vessels. We discovered that tumor cells invaded neighboring tissues and blood stream when primary tumors were at the microscopic sizes. These findings demonstrate that retinoblastoma metastasis occurs at the early stage and antiangiogenic drugs such as Vegf morpholino and sunitinib could potentially interfere with tumor invasiveness and metastasis. Thus, this orthotopic retinoblastoma model offers a new and unique opportunity to study the early events of tumor invasion, metastasis and drug responses.

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Citation

Scientific Reports, 2015, 5 : 10351

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Cardiovascular Sciences

Version

VoR (Version of Record)

Published in

Scientific Reports

Publisher

Nature Publishing Group

eissn

2045-2322

Acceptance date

08/04/2015

Copyright date

2015

Available date

27/01/2016

Publisher version

http://www.nature.com/articles/srep10351

Language

en

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