Investigating the role of pentraxin 3 as a biomarker for bacterial infection in subjects with COPD.pdf (677.97 kB)
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Investigating the role of pentraxin 3 as a biomarker for bacterial infection in subjects with COPD

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journal contribution
posted on 10.08.2017, 15:51 by Samantha J. Thulborn, Madiha Dilpazir, Koirobi Haldar, Vijay Mistry, Christopher E. Brightling, Michael R. Barer, Mona Bafadhel
BACKGROUND: Pentraxin 3 (PTX3) is an acute phase protein, involved in antibacterial resistance. Recent studies have shown PTX3 levels to be elevated in the presence of a bacterial infection and in a murine sepsis model. OBJECTIVE: We aim to investigate if sputum PTX3 can be used as a biomarker for bacterial infection in subjects with COPD. MATERIALS AND METHODS: Sputum samples from 142 COPD patients (102 men) with a mean (range) age of 69 years (45-85) and mean (SD) post-bronchodilator percentage predicted forced expiratory volume in 1 second (FEV1) of 50% (19) were analyzed for PTX3, using a commercial assay at stable state and during an exacerbation. Association with bacteria, from culture, quantitative real-time polymerase chain reaction (qPCR) and colony-forming units (CFU) was investigated. RESULTS: The geometric mean (95% CI) PTX3 level at stable state was 50.5 ng/mL (41.4-61.7). PTX3 levels correlated with absolute neutrophil count in sputum (r=0.37; P<0.01), but not FEV1 or health status. There was a weak correlation between PTX3 and bacterial load (CFU: r=0.29, P<0.01; 16S qPCR: r=0.18, P=0.05). PTX3 was a poor predictor of bacterial colonization (defined as >10(5) CFU/mL at stable state) with a receiver-operating characteristic (ROC) area under the curve (AUC) of 0.59 and 95% confidence interval (CI) 0.43-0.76 (P=0.21). During an exacerbation, there was a modest increase in PTX3 (fold difference 0.15, 95% of difference 0.02-0.29; P=0.02), and PTX3 fared better at identifying a bacteria-associated exacerbation (ROC AUC 0.65, 95% CI 0.52-0.78, P=0.03). CONCLUSION: PTX3 is associated with bacterial infection in patients with COPD, but its utility as a biomarker for identifying a bacteria-associated exacerbation warrants further studies.

History

Citation

International Journal of Chronic Obstructive Pulmonary Disease, 2017, 12, pp. 1199-1205

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammation

Version

VoR (Version of Record)

Published in

International Journal of Chronic Obstructive Pulmonary Disease

Publisher

Dove Medical Press

issn

1176-9106

eissn

1178-2005

Acceptance date

20/12/2016

Copyright date

2017

Available date

10/08/2017

Publisher version

https://www.dovepress.com/investigating-the-role-of-pentraxin-3-as-a-biomarker-for-bacterial-inf-peer-reviewed-article-COPD

Language

en

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