Investigating the structure of the factor B vWF-A domain?CD55 protein-protein complex using DEER spectroscopy: successes and pitfalls.pdf (435.96 kB)
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Investigating the structure of the factor B vWF-A domain/CD55 protein-protein complex using DEER spectroscopy: successes and pitfalls.

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posted on 24.10.2019, 10:19 by JE Lovett, RJM Abbott, P Roversi, S Johnson, JJE Caesar, M Doria, G Jeschke, CR Timmel, SM Lea
The electron paramagnetic resonance technique of double electron-electron resonance (DEER) was used to measure nanometre-scale distances between nitroxide spin labels attached to the complement regulatory protein CD55 (also known as decay accelerating factor) and the von Willebrand factor A (vWF-A) domain of factor B. Following a thorough assessment of the quality of the data, distances obtained from good-quality measurements are compared to predicted distances from a previously hypothesised model for the complex and are found to be incompatible. The success of using these distances as restraints in multi-body docking routines is presented critically.

Funding

We would like to thank Christian Bauer at MPI Mainz and Dr Jeff Harmer in Oxford (now at the University of Queensland) for EPR technical support. We thank Dr Jo Nettleship at the Oxford Protein Production Facility for mass spectrometry and Oxford Biochemistry DNA Sequencing Service for DNA sequencing. We gratefully acknowledge Prof. Axel Brunger at Stanford University for providing us with the CNS docking scripts and offering further support and advice. JEL thanks the Royal Society for a University Research Fellowship and University College Oxford for a Junior Research Fellowship. We thank the EPSRC for funding the Centre for Advanced Electron Spin Resonance and this work [grant number EP\DO48559\1]. The Wellcome Trust are thanked for funding to SML and her group.

History

Citation

Molecular Physics, 2013, 111 (18-19), pp. 2865-2872

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Molecular & Cell Biology

Version

VoR (Version of Record)

Published in

Molecular Physics

Publisher

Taylor & Francis

issn

0026-8976

Acceptance date

05/07/2013

Copyright date

2013

Available date

24/10/2019

Publisher version

https://www.tandfonline.com/doi/full/10.1080/00268976.2013.827754

Notes

Supplemental data for this article can be accessed online.

Language

en

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