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KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma.

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journal contribution
posted on 10.04.2018, 13:55 by A. G. Manente, G. Pinton, S. Zonca, D. Tavian, T. Habib, P. V. Jithesh, Dean Fennell, S. Nilsson, L. Moro
(Opening paragraph) AIM: To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM). MATERIALS & METHODS: We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines. RESULTS & CONCLUSION: We report a strong positive correlation between the expression of KDM6B and ERβ in MPM tumors and cell lines. We describe that, in hypoxia, the HIF2α-KDM6B axis induces an epithelioid morphology and ERβ expression in biphasic MPM cells with estrogen receptor-negative phenotype. Reduced histone H3K27 tri-methylation confirms KDM6B activity under hypoxic conditions. Importantly, cells treated during reoxygenation with the selective ERβ agonist, KB9520, maintain ERβ expression and the less aggressive phenotype acquired in hypoxia.

History

Citation

Epigenomics, 2016, 8 (9), pp. 1227-1238

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Cancer Research Centre

Version

VoR (Version of Record)

Published in

Epigenomics

Publisher

Future Medicine

issn

1750-1911

eissn

1750-192X

Copyright date

2016

Available date

10/04/2018

Publisher version

https://www.futuremedicine.com/doi/10.2217/epi-2016-0025

Language

en