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Laminin and fibronectin in rectal adenocarcinoma: Relationship to tumour grade, stage and metastasis

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posted on 09.12.2016, 11:55 by S. J. Forster, I. C . Talbot, D. R. Critchley
Using an immunoperoxidase procedure, we have examined the distribution of laminin and fibronectin in normal human large intestinal mucosa and in 50 cases of rectal adenocarcinoma for which extensive clinical follow up was available. In normal tissue, laminin staining was largely restricted to basement membranes, including that underlying the epithelial cells, whereas fibronectin was found in both basement membranes and surrounding connective tissue. In rectal carcinomas, basement membrane-like staining for laminin associated with tumour cells was found in only 27 out of the 50 cases studied. Statistical analysis showed that the presence of laminin-containing basement membranes was correlated with low histological grade (well-differentiated tumours), but not with stage (progression through the bowel wall and the development of lymph node metastases) and, in a highly significant way, with a reduced incidence of distant metastases and increased patient survival. Although fibronectin was found in tumour cell basement membranes where these were present, it was also found in the stroma of all 50 tumours. There was no apparent correlation between the presence of stromal fibronectin and grade, stage or development of metastases. Finally, attention is drawn to some of the technical difficulties in detecting basement membrane antigens in formalin-fixed tissue, the material most frequently available for retrospective study.

History

Citation

British Journal of Cancer, (1984) 50, 51–61.

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Molecular & Cell Biology

Version

VoR (Version of Record)

Published in

British Journal of Cancer

Publisher

Cancer Research UK, Nature Publishing Group

issn

0007-0920

eissn

1532-1827

Acceptance date

10/04/1984

Available date

09/12/2016

Publisher version

http://www.nature.com/bjc/journal/v50/n1/abs/bjc1984139a.html

Language

en

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