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Long Term Recordings with Immobile Silicon Probes in the Mouse Cortex

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posted on 12.06.2017, 14:24 by Michael Okun, Armin Lak, Matteo Carandini, Kenneth D. Harris
A key experimental approach in neuroscience involves measuring neuronal activity in behaving animals with extracellular chronic recordings. Such chronic recordings were initially made with single electrodes and tetrodes, and are now increasingly performed with high-density, high-count silicon probes. A common way to achieve long-term chronic recording is to attach the probes to microdrives that progressively advance them into the brain. Here we report, however, that such microdrives are not strictly necessary. Indeed, we obtained high-quality recordings in both head-fixed and freely moving mice for several months following the implantation of immobile chronic probes. Probes implanted into the primary visual cortex yielded well-isolated single units whose spike waveform and orientation tuning were highly reproducible over time. Although electrode drift was not completely absent, stable waveforms occurred in at least 70% of the neurons tested across consecutive days. Thus, immobile silicon probes represent a straightforward and reliable technique to obtain stable, long-term population recordings in mice, and to follow the activity of populations of well-isolated neurons over multiple days.

History

Citation

PLoS One, 2016, 11 (3): e0151180

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Neuroscience, Psychology and Behaviour

Version

VoR (Version of Record)

Published in

PLoS One

Publisher

Public Library of Science

eissn

1932-6203

Acceptance date

24/02/2016

Copyright date

2016

Available date

12/06/2017

Publisher version

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0151180

Language

en