Loss of Hsp70 exacerbates pathogenesis but not levels of fibrillar aggregates in a mouse model of Huntington's disease.
journal contributionposted on 24.10.2012, 08:59 by Jennifer L. Wacker, Shao-Yi Huang, Andrew D. Steele, Rebecca Aron, Gregor P. Lotz, QuangVu Nguyen, Flaviano Giorgini, Erik D. Roberson, Susan Lindquist, Eliezer Masliah, Paul J. Muchowski
Endogenous protein quality control machinery has long been suspected of influencing the onset and progression of neurodegenerative diseases characterized by accumulation of misfolded proteins. Huntington's disease (HD) is a fatal neurodegenerative disorder caused by an expansion of a polyglutamine (polyQ) tract in the protein huntingtin (htt), which leads to its aggregation and accumulation in inclusion bodies. Here, we demonstrate in a mouse model of HD that deletion of the molecular chaperones Hsp70.1 and Hsp70.3 significantly exacerbated numerous physical, behavioral and neuropathological outcome measures, including survival, body weight, tremor, limb clasping and open field activities. Deletion of Hsp70.1 and Hsp70.3 significantly increased the size of inclusion bodies formed by mutant htt exon 1, but surprisingly did not affect the levels of fibrillar aggregates. Moreover, the lack of Hsp70s significantly decreased levels of the calcium regulated protein c-Fos, a marker for neuronal activity. In contrast, deletion of Hsp70s did not accelerate disease in a mouse model of infectious prion-mediated neurodegeneration, ruling out the possibility that the Hsp70.1/70.3 mice are nonspecifically sensitized to all protein misfolding disorders. Thus, endogenous Hsp70s are a critical component of the cellular defense against the toxic effects of misfolded htt protein in neurons, but buffer toxicity by mechanisms independent of the deposition of fibrillar aggregates.
CitationJournal of Neuroscience, 2009, 29 (28), pp. 9104-9114
VersionVoR (Version of Record)
Published inJournal of Neuroscience
PublisherSociety for Neuroscience
Age FactorsAnalysis of VarianceAnimalsChromosomal ProteinsNon-HistoneDisease ModelsAnimalFemaleGene Expression RegulationHSP70 Heat-Shock ProteinsHSP72 Heat-Shock ProteinsHuntington DiseaseInclusion BodiesKaplan-Meier EstimateMaleMiceInbred C57BLKnockoutMotor ActivityMovement DisordersNerve Tissue ProteinsNeurologic ExaminationProto-Oncogene Proteins c-fosTrinucleotide Repeat ExpansionWeight Loss