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MADS-complexes regulate transcriptome dynamics during pollen maturation.

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journal contribution
posted on 24.10.2012, 08:57 by W. Verelst, David Twell, de Folter S., Richard Immink, H. Saedler, T. Münster
Differentiation processes are responsible for the diversity and functional specialization of the cell types that compose an organism. The outcome of these processes can be studied at molecular, physiologic, and biochemical levels by comparing different cell types, but the complexity and dynamics of the regulatory processes that specify the differentiation are largely unexplored. Background Differentiation processes are responsible for the diversity and functional specialization of the cell types that compose an organism. The outcome of these processes can be studied at molecular, physiologic, and biochemical levels by comparing different cell types, but the complexity and dynamics of the regulatory processes that specify the differentiation are largely unexplored. Results Here we identified the pollen-specific MIKC* class of MADS-domain transcription factors as major regulators of transcriptome dynamics during male reproductive cell development in Arabidopsis thaliana. Pollen transcript profiling of mutants deficient in different MIKC* protein complexes revealed that they control a transcriptional switch that directs pollen maturation and that is essential for pollen competitive ability. We resolved the functional redundancy among the MIKC* proteins and uncovered part of the underlying network by identifying the non-MIKC* MADS-box genes AGL18 and AGL29 as downstream regulators of a subset of the MIKC* MADS-controlled genes. Conclusion Our results provide a first, unique, and compelling insight into the complexity of a transcription factor network that directs cellular differentiation during pollen maturation, a process that is essential for male reproductive fitness in flowering plants.

Funding

WV and TM thank the Collaborative Research Centre 680 (Deutsche Forschungsgemeinschaft) for funding the microarray experiments, SdF and RI thank The Netherlands Proteomics Centre (NPC) for funding, and DT gratefully acknowledges financial support from The Royal Society and the Biotechnology and Biological Sciences Research Council.

History

Citation

Genome Biology, 2007, 8 (11), pp. R249-R249

Published in

Genome Biology

Publisher

BioMed Central

eissn

1465-6914

Copyright date

2007

Available date

24/10/2012

Publisher version

http://genomebiology.com/content/8/11/R249

Language

eng