10.1038_CDDIS.2013.76.pdf (1.02 MB)
Download file

Metabolic effects of TiO₂ nanoparticles, a common component of sunscreens and cosmetics, on human keratinocytes

Download (1.02 MB)
journal contribution
posted on 19.11.2013, 14:45 by P. Tucci, G. Porta, M. Agostini, D. Dinsdale, I. Iavicoli, K. Cain, A. Finazzi-Agro, G. Melino, A. Willis
The long-term health risks of nanoparticles remain poorly understood, which is a serious concern given their prevalence in the environment from increased industrial and domestic use. The extent to which such compounds contribute to cellular toxicity is unclear, and although it is known that induction of oxidative stress pathways is associated with this process, the proteins and the metabolic pathways involved with nanoparticle-mediated oxidative stress and toxicity are largely unknown. To investigate this problem further, the effect of TiO[subscript 2] on the HaCaT human keratinocyte cell line was examined. The data show that although TiO[subscript 2] does not affect cell cycle phase distribution, nor cell death, these nanoparticles have a considerable and rapid effect on mitochondrial function. Metabolic analysis was performed to identify 268 metabolites of the specific pathways involved and 85 biochemical metabolites were found to be significantly altered, many of which are known to be associated with the cellular stress response. Importantly, the uptake of nanoparticles into the cultured cells was restricted to phagosomes, TiO[subscript 2] nanoparticles did not enter into the nucleus or any other cytoplasmic organelle. No other morphological changes were detected after 24-h exposure consistent with a specific role of mitochondria in this response.



Cell Death and Disease ,2013 ,4, e549


VoR (Version of Record)

Published in

Cell Death and Disease


Nature Publishing Group for Associazione Differenziamento e Morte Cellulare



Copyright date


Available date


Publisher version




Usage metrics