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Metabolic molecular markers of the tidal clock in the marine crustacean Eurydice pulchra

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posted on 18.02.2016, 11:10 by J. S. O'Neill, Kate D. Lee, Lin Zhang, K. Feeney, S. G. Webster, Matthew James Blades, Charalambos P. Kyriacou, M. H. Hastings, D. C. Wilcockson
In contrast to the well mapped molecular orchestration of circadian timekeeping in terrestrial organisms, the mechanisms that direct tidal and lunar rhythms in marine species are entirely unknown. Using a combination of biochemical and molecular approaches we have identified a series of metabolic markers of the tidal clock of the intertidal isopod Eurydice pulchra. Specifically, we show that the overoxidation of peroxiredoxin (PRX), a conserved marker of circadian timekeeping in terrestrial eukaryotes [1], follows a circatidal (approximately 12.4 hours) pattern in E. pulchra, in register with the tidal pattern of swimming. In parallel, we show that mitochondrially encoded genes are expressed with a circatidal rhythm. Together, these findings demonstrate that PRX overoxidation rhythms are not intrinsically circadian; rather they appear to resonate with the dominant metabolic cycle of an organism, regardless of its frequency. Moreover, they provide the first molecular leads for dissecting the tidal clockwork.

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Citation

Current Biology , 2015, 25 (8), pp. R326-R327

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Department of Genetics

Version

VoR (Version of Record)

Published in

Current Biology

Publisher

Elsevier (Cell Press)

issn

0960-9822

eissn

1879-0445

Copyright date

2015

Available date

18/02/2016

Publisher version

http://www.sciencedirect.com/science/article/pii/S0960982215002213

Language

en

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