Method for inducing experimental pneumococcal meningitis in outbred mice.pdf (1.67 MB)
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Method for inducing experimental pneumococcal meningitis in outbred mice.

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journal contribution
posted on 18.10.2019, 13:57 by Damiana Chiavolini, Sergio Tripodi, Riccardo Parigi, Marco R. Oggioni, Elisabetta Blasi, Marcella Cintorino, Gianni Pozzi, Susanna Ricci
BACKGROUND: Streptococcus pneumoniae is the leading cause of bacterial meningitis. Pneumococcal meningitis is associated with the highest mortality among bacterial meningitis and it may also lead to neurological sequelae despite the use of antibiotic therapy. Experimental animal models of pneumococcal meningitis are important to study the pathogenesis of meningitis, the host immune response induced after infection, and the efficacy of novel drugs and vaccines. RESULTS: In the present work, we describe in detail a simple, reproducible and efficient method to induce pneumococcal meningitis in outbred mice by using the intracranial subarachnoidal route of infection. Bacteria were injected into the subarachnoid space through a soft point located 3.5 mm rostral from the bregma. The model was tested with several doses of pneumococci of three capsular serotypes (2, 3 and 4), and mice survival was recorded. Lethal doses killing 50 % of animals infected with type 2, 3 and 4 S. pneumoniae were 3.2 x 10, 2.9 x 10 and 1.9 x 10(2) colony forming units, respectively. Characterisation of the disease caused by the type 4 strain showed that in moribund mice systemic dissemination of pneumococci to blood and spleen occurred. Histological analysis of the brain of animals infected with type 4 S. pneumoniae proved the induction of meningitis closely resembling the disease in humans. CONCLUSIONS: The proposed method for inducing pneumococcal meningitis in outbred mice is easy-to-perform, fast, cost-effective, and reproducible, irrespective of the serotype of pneumococci used.

Funding

The work was supported by the Commission of the European Union (contract QLK2-2000-01536) and MIUR (COFIN 2002). We would like to acknowledge Velia Braione for excellent technical assistance with experimental work.

History

Citation

BMC Microbiolog, 2004, volume 4, Article number: 36

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Genetics and Genome Biology

Version

VoR (Version of Record)

Published in

BMC Microbiolog

Publisher

BMC (part of Springer Nature)

eissn

1471-2180

Acceptance date

22/09/2004

Copyright date

2004

Available date

18/10/2019

Publisher version

https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-4-36

Notes

Additional File 1 Document stating the ethical approval for animal experimentation conceded to the Laboratory of Molecular Microbiology and Biotechnology (LA.M.M.B.) from the University Hospital of Siena, the Medical Faculty and the Local Ethical Committee (document no. 754/03, 12.9.03). [http://www.biomedcentral.com/content/supplementary/1471- 2180-4-36-S1.pdf]

Language

en