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Mice carrying a complete deletion of the talin2 coding sequence are viable and fertile.

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journal contribution
posted on 07.11.2016, 15:12 by Emmanuel Debrand, Francesco J. Conti, Neil Bate, Lorraine Spence, Daniela Mazzeo, Catrin A. Pritchard, Susan J. Monkley, David R. Critchley
Mice homozygous for several Tln2 gene targeted alleles are viable and fertile. Here we show that although the expression of talin2 protein is drastically reduced in muscle from these mice, other tissues continue to express talin2 albeit at reduced levels. We therefore generated a Tln2 allele lacking the entire coding sequence (Tln2(cd)). Tln2(cd/cd) mice were viable and fertile, and the genotypes of Tln2(cd/+) intercrosses were at the expected Mendelian ratio. Tln2(cd/cd) mice showed no major difference in body mass or the weight of the major organs compared to wild-type, although they displayed a mildly dystrophic phenotype. Moreover, Tln2(cd/cd) mouse embryo fibroblasts showed no obvious defects in cell adhesion, migration or proliferation. However, the number of Tln2(cd/cd) pups surviving to adulthood was variable suggesting that such mice have an underlying defect.

Funding

The Wellcome Trust and Cancer Research UK supported this work.

History

Citation

Biochemical and Biophysical Research Communications , 2012, 426 (2), pp. 190-195

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Cancer Studies and Molecular Medicine

Version

VoR (Version of Record)

Published in

Biochemical and Biophysical Research Communications

Publisher

Elsevier for Academic Press

issn

0006-291X

eissn

1090-2104

Available date

07/11/2016

Publisher version

http://www.sciencedirect.com/science/article/pii/S0006291X12015719

Language

en