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Microsatellite variation of ESR1, ESR2, and AR in Serbian women with primary ovarian insufficiency

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journal contribution
posted on 08.02.2019, 12:48 by J Li, R Dalgleish, S Vujovic, S Dragojevic-Dikic, M Ivanisevic, M Ivovic, M Tancic, J Thompson, F Al-Azzawi
OBJECTIVE: This study aimed to investigate the potential role of microsatellite polymorphisms of the estrogen receptor alpha gene (ESR1) TA repeat, estrogen receptor beta gene (ESR2) CA repeat, and androgen receptor gene (AR) CAG and GGN repeats among Serbian women with primary ovarian insufficiency (POI). These microsatellites have been reported to be associated with POI in different racial/ethnic populations. METHODS: A cohort of 196 POI cases matched with 544 fertile controls was recruited by the Institute for Endocrinology, Diabetes and Metabolic Disorders of Serbia between 2007 and 2010. DNA was extracted from saliva. The four microsatellites were genotyped using a PCR-based assay to determine the repeat lengths. RESULTS: POI patients carried shorter repeat lengths of ESR2 (CA)n than controls (P = 0.034), but the difference was small. ESR1 (TA)n was on the borderline of statistical differences between groups (P = 0.059). AR (CAG)n and (GGN)n showed no association with POI. CONCLUSIONS: We cautiously conclude that microsatellite polymorphisms of gonadal steroid receptor genes might contribute to the genetic basis of POI in Serbian women, but a larger-scale study and family-based studies are warranted to validate our findings even though the sample size in this study is larger than any previously published in this field.

Funding

This study was supported by a grant from the Institute of Women’s Health Charity, Leicester, UK.

History

Citation

Climacteric, 2018, 21 (5), pp. 472-477

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Genetics and Genome Biology

Version

AM (Accepted Manuscript)

Published in

Climacteric

Publisher

Taylor & Francis

eissn

1473-0804

Acceptance date

03/05/2018

Copyright date

2018

Available date

29/08/2019

Publisher version

https://www.tandfonline.com/doi/full/10.1080/13697137.2018.1476967

Notes

The file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.

Language

en