Optimisation of the synthesis of vancomycin-selective molecularly imprinted polymer nanoparticles using automatic photoreactor
journal contributionposted on 10.07.2015, 11:10 by K. Muzyka, Khalku Karim, Antonio Guerreiro, Alessandro Poma, Sergey Piletsky
A novel optimized protocol for solid-state synthesis of molecularly imprinted polymer nanoparticles (nanoMIPs) with specificity for antibiotic vancomycin is described. The experimental objective was optimization of the synthesis parameters (factors) affecting the yield of obtained nanoparticles which have been synthesized using the first prototype of an automated solid-phase synthesizer. Applications of experimental design (or design of experiments) in optimization of nanoMIP yield were carried out using MODDE 9.0 software. The factors chosen in the model were the amount of functional monomers in the polymerization mixture, irradiation time, temperature during polymerization, and elution temperature. In general, it could be concluded that the irradiation time is the most important and the temperature was the least important factor which influences the yield of nanoparticles. Overall, the response surface methodology proved to be an effective tool in reducing time required for optimization of complex experimental conditions.
CitationNanoscale Research Letters 2014, 9 : 154
Author affiliation/Organisation/COLLEGE OF SCIENCE AND ENGINEERING/Department of Chemistry
VersionVoR (Version of Record)
Published inNanoscale Research Letters 2014
Science & TechnologyTechnologyPhysical SciencesNanoscience & NanotechnologyMaterials Science, MultidisciplinaryPhysics, AppliedScience & Technology - Other TopicsMaterials SciencePhysicsMATERIALS SCIENCE, MULTIDISCIPLINARYNANOSCIENCE & NANOTECHNOLOGYPHYSICS, APPLIEDDesign of experimentsMolecularly imprinted polymerNanoparticlesImmuno-like assayAutomatic photoreactorOptimizationMODDEPERFORMANCEDESIGN