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Overexpression of TWO-IN-ONE Domains Inhibits Cytokinesis in Arabidopsis

journal contribution
posted on 22.06.2022, 11:04 authored by SA Oh, SJ Lee, JH Kim, D Twell, SK Park

Plant cells undergoing cytokinesis build a cell plate between daughter nuclei and expand it centrifugally toward the parental plasma membrane. This process is enabled by the phragmoplast, a plant-specific and highly dynamic microtubule (MT)-based array. A signaling module required for phragmoplast expansion consisting of TWO-IN-ONE (TIO) and two Kinesin-12 members is described in Arabidopsis thaliana. TIO is a deeply conserved Fused kinase that has a divergent plant-specific role in cytokinesis. TIO has two conserved domains, the N-terminal kinase and the C-terminal ARM/HEAT domains, which are crucial for function and interaction with the phragmoplast MT plus-end associated Kinesin-12A and -12B. Here, we further explored the importance of TIO domains for cytokinesis in an overexpression approach. In addition, the functionality of a putative activation loop in the kinase domain was validated by substituting two invariant threonine and serine residues. Phenotypic analysis reveals that overexpression of the domains severely interferes with cytokinesis in pollen and in somatic cells. Furthermore, measuring the level of rescue for gametophytic cytokinesis in tio-3 null mutant background reveals that the putative kinase activation loop is required for TIO function and likely operates via reversible phosphorylations. Therefore, this study enhances understanding of TIO signaling and domain functions in cytokinesis.

Funding

This work was supported by a grant to S.K.P (NRF-2021R1A2C2010124) from the National Research Foundation (NRF), Ministry of Education, Science and Technology in the Republic of Korea and support to DT (BB/E001017/1; BB/I011269/1) from the UK Biotechnology and Biological Sciences Research Council (BBSRC).

History

Citation

J. Plant Biol. (2022). https://doi.org/10.1007/s12374-022-09353-6

Author affiliation

Department of Genetics and Genome Biology, University of Leicester

Version

AM (Accepted Manuscript)

Published in

Journal of Plant Biology

Publisher

Springer Science and Business Media LLC

issn

1226-9239

eissn

1867-0725

Acceptance date

27/04/2022

Copyright date

2022

Available date

25/05/2023

Language

en