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Proinsulin C-Peptide Enhances Cell Survival and Protects against Simvastatin-Induced Myotoxicity in L6 Rat Myoblasts.

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journal contribution
posted on 24.06.2019, 10:48 by Sumia Mohamed Essid, Alan Bevington, Nigel J. Brunskill
The repair capacity of progenitor skeletal muscle satellite cells (SC) in Type 1 diabetes mellitus (T1DM) is decreased. This is associated with the loss of skeletal muscle function. In T1DM, the deficiency of C-peptide along with insulin is associated with an impairment of skeletal muscle functions such as growth, and repair, and is thought to be an important contributor to increased morbidity and mortality. Recently, cholesterol-lowering drugs (statins) have also been reported to increase the risk of skeletal muscle dysfunction. We hypothesised that C-peptide activates key signaling pathways in myoblasts, thus promoting cell survival and protecting against simvastatin-induced myotoxicity. This was tested by investigating the effects of C-peptide on the L6 rat myoblast cell line under serum-starved conditions. Results: C-peptide at concentrations as low as 0.03 nM exerted stimulatory effects on intracellular signaling pathways-MAP kinase (ERK1/2) and Akt. When apoptosis was induced by simvastatin, 3 nM C-peptide potently suppressed the apoptotic effect through a pertussis toxin-sensitive pathway. Simvastatin strongly impaired Akt signaling and stimulated the reactive oxygen species (ROS) production; suggesting that Akt signaling and oxidative stress are important factors in statin-induced apoptosis in L6 myoblasts. The findings indicate that C-peptide exerts an important protective effect against death signaling in myoblasts. Therefore, in T1DM, the deficiency of C-peptide may contribute to myopathy by rendering myoblast-like progenitor cells (involved in muscle regeneration) more susceptible to the toxic effects of insults such as simvastatin.

Funding

This research was supported by the Ministry of Higher Education and Scientific Research, Libya.

History

Citation

International Journal of Molecular Sciences, 2019, 20 (7)

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Themes/Molecular & Cellular Bioscience

Version

VoR (Version of Record)

Published in

International Journal of Molecular Sciences

Publisher

MDPI

eissn

1422-0067

Acceptance date

25/03/2019

Copyright date

2019

Available date

24/06/2019

Publisher version

https://www.mdpi.com/1422-0067/20/7/1654

Language

en

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