Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria.pdf (3.86 MB)
Download file

Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria.

Download (3.86 MB)
journal contribution
posted on 11.01.2018, 09:47 by Rahul D. Kaduskar, Giulia Della Scala, Zaaima J. H. Al Jabri, Stefania Arioli, Loana Musso, Marco R. Oggioni, Sabrina Dallavalle, Diego Mora
Promysalin was previously described as a narrow spectrum molecule with a unique species-specific activity against Pseudomonas aeruginosa. Here we demonstrate that promysalin is active against Gram-positive and Gram-negative bacteria using a microdilution assay. Promysalin acts on Gram-positive bacteria with a mechanism of action involving cell membrane damage with leakage of intracellular components. The evaluation of MICs and MBCs on 11 promysalin analogs, synthesized utilizing diverted total synthesis, allowed the identification of the structural moieties potentially involved in cell membrane interaction and damage. The mechanism of action of promysalin against Gram-negative bacteria is still not clarified, even if a synergistic effect with the bisguanidine chlorhexidine on cell membrane disruption has been observed.

Funding

We acknowledge Professor René De Mot, Centre of Microbial and Plant Genetics, Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium, for providing us P. putida RW10S1 and P. stutzeri LMG 2333.

History

Citation

Scientific Reports, 2017, 7:8861

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Genetics and Genome Biology

Version

VoR (Version of Record)

Published in

Scientific Reports

Publisher

Nature Publishing Group

issn

2045-2322

eissn

2045-2322

Acceptance date

28/06/2017

Copyright date

2017

Available date

11/01/2018

Publisher version

https://www.nature.com/articles/s41598-017-07567-0

Notes

Supplementary information accompanies this paper at doi:10.1038/s41598-017-07567-0

Language

en

Usage metrics

Categories

Keywords

Licence

Exports