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Quantitative studies of pinocytosis. I. Kinetics of uptake of (125I)polyvinylpyrrolidone by rat yolk sac cultured in vitro

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journal contribution
posted on 04.03.2016, 10:16 by Kenneth E. Williams, Elizabeth M. Kidston, Felix Beck, John B. Lloyd
A method is described for the in vitro culture of 17.5-day rat visceral yolk sac. Tissue survival was good as judged by light and electron microscopy. The rate of pinocytic uptake of 125I-labeled polyvinylpyrrolidone by the tissue was constant both within and between experiments. Within the concentration range 0.15-24 mug/ml, the 125I-labeled polyvinylpyrrolidone neither stimulated nor inhibited pinocytosis. The system offers many advantages in the quantitative study of the physical basis of pinocytosis.

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This work was supported by a generous grant from Tenovus.

History

Citation

The Journal of Cell Biology, 2003 vol. 64 no. 1 113-122

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Molecular & Cell Biology

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VoR (Version of Record)

Published in

The Journal of Cell Biology

Publisher

Rockefeller University Press

issn

0021-9525

eissn

1540-8140

Available date

04/03/2016

Publisher version

http://jcb.rupress.org/content/64/1/113

Language

en

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