Recent advances in pericentriolar material organization: ordered layers and scaffolding gels.pdf (1.1 MB)
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Recent advances in pericentriolar material organization: ordered layers and scaffolding gels.

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journal contribution
posted on 04.05.2018, 14:30 by Andrew M. Fry, Josephina Sampson, Caroline Shak, Sue Shackleton
The centrosome is an unusual organelle that lacks a surrounding membrane, raising the question of what limits its size and shape. Moreover, while electron microscopy (EM) has provided a detailed view of centriole architecture, there has been limited understanding of how the second major component of centrosomes, the pericentriolar material (PCM), is organized. Here, we summarize exciting recent findings from super-resolution fluorescence imaging, structural biology, and biochemical reconstitution that together reveal the presence of ordered layers and complex gel-like scaffolds in the PCM. Moreover, we discuss how this is leading to a better understanding of the process of microtubule nucleation, how alterations in PCM size are regulated in cycling and differentiated cells, and why mutations in PCM components lead to specific human pathologies.

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Citation

F1000Research, 2017, 6 (F1000 Faculty Rev), 1622

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Molecular & Cell Biology

Version

VoR (Version of Record)

Published in

F1000Research

issn

2046-1402

Copyright date

2017

Available date

04/05/2018

Publisher version

https://f1000research.com/articles/6-1622/v1

Language

en

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