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Reproducibility of telomere length assessment: an international collaborative study

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journal contribution
posted on 24.02.2016, 09:46 by C. M. Martin-Ruiz, D. Baird, L. Roger, P. Boukamp, D. Krunic, R. Cawthon, M. M. Dokter, P. van der Harst, S. Bekaert, T. de Meyer, G. Roos, U. Svenson, Veryan Codd, Nilesh Jayantilal Samani, L. McGlynn, P. G. Shiels, K. A. Pooley, A. M. Dunning, R. Cooper, A. Wong, A. Kingston, T. von Zglinicki
BACKGROUND: Telomere length is a putative biomarker of ageing, morbidity and mortality. Its application is hampered by lack of widely applicable reference ranges and uncertainty regarding the present limits of measurement reproducibility within and between laboratories. METHODS: We instigated an international collaborative study of telomere length assessment: 10 different laboratories, employing 3 different techniques [Southern blotting, single telomere length analysis (STELA) and real-time quantitative PCR (qPCR)] performed two rounds of fully blinded measurements on 10 human DNA samples per round to enable unbiased assessment of intra- and inter-batch variation between laboratories and techniques. RESULTS: Absolute results from different laboratories differed widely and could thus not be compared directly, but rankings of relative telomere lengths were highly correlated (correlation coefficients of 0.63-0.99). Intra-technique correlations were similar for Southern blotting and qPCR and were stronger than inter-technique ones. However, inter-laboratory coefficients of variation (CVs) averaged about 10% for Southern blotting and STELA and more than 20% for qPCR. This difference was compensated for by a higher dynamic range for the qPCR method as shown by equal variance after z-scoring. Technical variation per laboratory, measured as median of intra- and inter-batch CVs, ranged from 1.4% to 9.5%, with differences between laboratories only marginally significant (P = 0.06). Gel-based and PCR-based techniques were not different in accuracy. CONCLUSIONS: Intra- and inter-laboratory technical variation severely limits the usefulness of data pooling and excludes sharing of reference ranges between laboratories. We propose to establish a common set of physical telomere length standards to improve comparability of telomere length estimates between laboratories.

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Citation

International Journal of Epidemiology, 2015, 44 (5), pp. 1673-1683

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Cardiovascular Sciences

Version

VoR (Version of Record)

Published in

International Journal of Epidemiology

Publisher

Oxford University Press (OUP)

issn

0300-5771

eissn

1464-3685

Acceptance date

28/08/2014

Copyright date

2014

Available date

24/02/2016

Publisher version

http://ije.oxfordjournals.org/content/44/5/1673

Language

en

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