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Rhythmic expression of the cycle gene in a hematophagous insect vector.

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journal contribution
posted on 24.10.2012, 09:12 by A.C. Meireles-Filho, P.R. Amoretty, N.A. Souza, Charalambos P. Kyriacou, A.A. Peixoto
Background: A large number of organisms have internal circadian clocks that enable them to adapt to the cyclic changes of the external environment. In the model organism Drosophila melanogaster, feedback loops of transcription and translation are believed to be crucial for the maintenance of the central pacemaker. In this mechanism the cycle (or bmal1) gene, which is constitutively expressed, plays a critical role activating the expression of genes that will later inhibit their own activity, thereby closing the loop. Unlike Drosophila, the molecular clock of insect vectors is poorly understood, despite the importance of circadian behavior in the dynamic of disease transmission. Results: Here we describe the sequence, genomic organization and circadian expression of cycle in the crepuscular/nocturnal hematophagous sandfly Lutzomyia longipalpis, the main vector of visceral leishmaniasis in the Americas. Deduced amino acid sequence revealed that sandfly cycle has a C-terminal transactivation domain highly conserved among eukaryotes but absent in D. melanogaster. Moreover, an alternative form of the transcript was also identified. Interestingly, while cycle expression in Drosophila and other Diptera is constitutive, in sandflies it is rhythmic in males and female heads but constitutive in the female body. Blood-feeding, which causes down-regulation of period and timeless in this species, does not affect cycle expression. Conclusion: Sequence and expression analysis of cycle in L. longipalpis show interesting differences compared to Drosophila suggesting that hematophagous vector species might present interesting new models to study the molecular control of insect circadian clocks.

History

Citation

BMC Molecular Biology, 2006, 7:38

Version

VoR (Version of Record)

Published in

BMC Molecular Biology

Publisher

BioMed Central Ltd

eissn

1471-2199

Copyright date

2006

Available date

24/10/2012

Publisher version

http://www.biomedcentral.com/1471-2199/7/38

Language

eng