Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle.pdf (9.16 MB)
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Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle.

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journal contribution
posted on 24.07.2019, 14:24 by AJ Capel, RP Rimington, JW Fleming, DJ Player, LA Baker, MC Turner, JM Jones, NRW Martin, RA Ferguson, VC Mudera, MP Lewis
Tissue engineered skeletal muscle allows investigation of the cellular and molecular mechanisms that regulate skeletal muscle pathology. The fabricated model must resemble characteristics of in vivo tissue and incorporate cost-effective and high content primary human tissue. Current models are limited by low throughput due to the complexities associated with recruiting tissue donors, donor specific variations, as well as cellular senescence associated with passaging. This research presents a method using fused deposition modeling (FDM) and laser sintering (LS) 3D printing to generate reproducible and scalable tissue engineered primary human muscle, possessing aligned mature myotubes reminiscent of in vivo tissue. Many existing models are bespoke causing variability when translated between laboratories. To this end, a scalable model has been developed (25-500 μL construct volumes) allowing fabrication of mature primary human skeletal muscle. This research provides a strategy to overcome limited biopsy cell numbers, enabling high throughput screening of functional human tissue.

Funding

The authors would like to acknowledge Loughborough University, EPSRC (grant reference EP/L02067X/1) for funding and support for this work. In addition, this research was funded by the NIHR Leicester Biomedical Research Centre.

History

Citation

Frontiers in Bioengineering and Biotechnology, 2019, 7:20

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Department of Infection, Immunity and Inflammation

Version

VoR (Version of Record)

Published in

Frontiers in Bioengineering and Biotechnology

Publisher

Frontiers Media

issn

2296-4185

Acceptance date

28/01/2019

Copyright date

2019

Available date

24/07/2019

Publisher version

https://www.frontiersin.org/articles/10.3389/fbioe.2019.00020/full

Notes

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fbioe.2019.00020/full#supplementary-material

Language

en