Acetate_promotes_adipogenesis_in_brown_adipocytes_Endocrinology_May_2016_Kyrou_Randeva_accepted_pdf_prior_to_publishing.pdf (727.73 kB)
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Short-Chain Fatty Acid Acetate Stimulates Adipogenesis and Mitochondrial Biogenesis via GPR43 in Brown Adipocytes.

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journal contribution
posted on 24.04.2019, 14:32 by J Hu, I Kyrou, BK Tan, GK Dimitriadis, M Ramanjaneya, G Tripathi, V Patel, S James, M Kawan, J Chen, HS Randeva
Short-chain fatty acids play crucial roles in a range of physiological functions. However, the effects of short-chain fatty acids on brown adipose tissue have not been fully investigated. We examined the role of acetate, a short-chain fatty acid formed by fermentation in the gut, in the regulation of brown adipocyte metabolism. Our results show that acetate up-regulates adipocyte protein 2, peroxisomal proliferator-activated receptor-γ coactivator-1α, and uncoupling protein-1 expression and affects the morphological changes of brown adipocytes during adipogenesis. Moreover, an increase in mitochondrial biogenesis was observed after acetate treatment. Acetate also elicited the activation of ERK and cAMP response element-binding protein, and these responses were sensitive to G(i/o)-type G protein inactivator, Gβγ-subunit inhibitor, phospholipase C inhibitor, and MAPK kinase inhibitor, indicating a role for the G(i/o)βγ/phospholipase C/protein kinase C/MAPK kinase signaling pathway in these responses. These effects of acetate were mimicked by treatment with 4-chloro-α-(1-methylethyl)-N-2-thiazolylbenzeneacetamide, a synthetic G protein-coupled receptor 43 (GPR43) agonist and were impaired in GPR43 knockdown cells. Taken together, our results indicate that acetate may have important physiological roles in brown adipocytes through the activation of GPR43.

Funding

H.S.R. acknowledges funding support from the General Charities of the City of Coventry and is grateful to Dr Mark Christian (University of Warwick, Coventry, United Kingdom) for providing the IM-BAT cell line and to Professor Bo Bai (Jining University) for providing assistance for the animal experiments.

History

Citation

Endocrinology, 2016, 157 (5), pp. 1881-1894

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Department of Cardiovascular Sciences

Version

AM (Accepted Manuscript)

Published in

Endocrinology

Publisher

Oxford University Press (OUP), Endocrine Society

eissn

1945-7170

Acceptance date

14/03/2016

Copyright date

2016

Available date

24/04/2019

Publisher version

https://academic.oup.com/endo/article/157/5/1881/2422634

Language

en