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Short communication: Towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the elaeis genus

journal contribution
posted on 24.04.2018, 13:13 by Noorharzia Mohd Zaki, Rajinder Singh, Nordiana Hanim Mohd Nor, Muhammad Azwan Zulkifli, Steven W. Smith, Trude Schwarzacher, M. Madon, J. S. Heslop-Harrison
The development of Elaeis guineensis chromosome-arm specific markers is required for linking the genetic, sequence and chromosomal maps, valuable for comparative studies, hybridisation and breeding. Here, we aimed to develop strategies exploiting assembled short shotgun-sequence reads to identify markers for in situ hybridisation to identify arms and large-scale organisation of pseudo-chromosome 1. The conserved putative repetitive DNA sequence found through informatics analysis showed an extra intercalary band in one arm of pseudo-chromosome 1 in fluorescence the in situ hybridisation result. The results are a basis for establishing a North-South orientation of E. guineensis pseudo-chromosome. Furthermore, the ability of the newly developed markers for distinguishing both Elaeis species with in situ hybridisation showed their utility in identifying Elaeis hybrids.

Funding

The first author would like to thank MPOB for Ph.D fellowship.

History

Citation

Journal of Oil Palm Research, 2017, 29 (4), pp. 594-599

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Old Departments Pre 01 Aug 2015/Department of Biology (Pre 01 Aug 2015)

Version

AM (Accepted Manuscript)

Published in

Journal of Oil Palm Research

Publisher

Malaysian Palm Oil Board

issn

1511-2780

Acceptance date

07/09/2017

Copyright date

2017

Publisher version

http://jopr.mpob.gov.my/short-communication-towards-development-of-elaeis-guineensis-chromosome-arm-specific-markers-and-their-utility-across-the-elaeis-genus/

Notes

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Language

en