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Simple High-Resolution NMR Spectroscopy as A Tool in Molecular Biology

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journal contribution
posted on 16.08.2019, 16:46 by Luca Mureddu, Geerten W. Vuister
NMR is one of the major techniques for investigating the structure, dynamics and interactions between biomolecules. However, non‐experts often experience NMR experimentation and data analysis as intimidating. We discuss a simple yet powerful NMR technique, the so‐called chemical shift perturbation (CSP) analysis, as a tool to elucidate macromolecular interactions in small‐ and medium‐sized complexes, including protein‐protein, protein‐drug, and protein‐DNA/RNA interactions. We discuss current software packages for NMR data analysis and present a new interactive graphical tool implemented in CcpNmr AnalysisAssign version‐3, which can drastically reduce the time required for the CSP analysis. Lastly, we illustrate the usefulness of a protein three‐dimensional structure for interpretation of the CSP data.

Funding

We thank Drs Cyril Dominguez and Mikael Feracci for the usage of their Tstar‐KH/Clip2 data. We also thank Drs Ed Brooksbank, Wayne Boucher and Rasmus Fogh for their expert contributions to the CcpNmr AnalysisAssign programme. We thank the editors for helpful comments. LM acknowledges his stipend provided by MRC‐IMPACT PhD programme (grant MR/NO13913/1) and GWV acknowledges funding of the CCPN project by MRC (grants MR/L000555/1 and MR/P00038X/1).

History

Citation

FEBS Journal, 2019, 286(11), pp. 2035-2042

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Molecular & Cell Biology

Version

VoR (Version of Record)

Published in

FEBS Journal

Publisher

Wiley, Federation of European Biochemical Societies

issn

0014-2956

Acceptance date

30/01/2019

Copyright date

2019

Available date

16/08/2019

Publisher version

https://febs.onlinelibrary.wiley.com/doi/full/10.1111/febs.14771

Notes

AnalysisAssign can be freely downloaded for non‐commercial academic usage from http://www.ccpn.ac.uk/v3-software/downloads. The CSP module and tutorials will be included in its upcoming version 3.0 release.

Language

en

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