Sputum Moraxella catarrhalis strains exhibit diversity within and between COPD subjects. .pdf (653.02 kB)
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Sputum Moraxella catarrhalis strains exhibit diversity within and between COPD subjects.

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posted on 12.08.2019, 15:19 by LM George, RD Haigh, V Mistry, K Haldar, MR Barer, MR Oggioni, CE Brightling
Purpose: Moraxella catarrhalis is implicated in the pathogenesis of some COPD exacerbations. We sought to investigate whether the M. catarrhalis strain is variable between COPD subjects; that an exacerbation is associated with acquisition of a new strain and that certain strains are more commonly associated with exacerbations. Patients and methods: Sputum samples were collected at stable and exacerbation visits from COPD subjects from a single center as part of the COPDMAP consortium. Samples identified as M. catarrhalis positive by qPCR were recultured in liquid cultures grown to extract genomic DNA; underwent Illumina MiSeq and bacterial genome sequences were de novo assembled and Multi Locus Sequence Type (MLST) was determined. Results: Thirty-five samples were obtained from 18 subjects. These included 13 stable and 22 exacerbation samples. The diversity between samples was very large with 25 different M. catarrhalis MLSTs being identified out of the 35 samples of which 12 MSLTs have not been described previously. Change and persistence of M. catarrhalis strain were observed between stable visits, from stable to exacerbation and vice-a-versa, and between exacerbation visits. Conclusion: Sputum M. catarrhalis strains exhibit marked diversity within and between COPD subjects. Acquisition of a new strain is common between stable and exacerbation events such that no strain is specifically associated with an exacerbation.

Funding

This work was funded in part by Airway Disease Predicting Outcomes through Patient Specific Computational Modeling (AirPROM) project (funded through FP7 EU grant), National Institute for Health Research (NIHR) Leicester Respiratory Biomedical Center and MRC UK (COPDMAP). This paper presents independent research funded by the NIHR.

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Citation

International Journal of Chronic Obstructive Pulmonary Disease, 2018, 13, pp. 3663-3667

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Department of Infection, Immunity and Inflammation

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VoR (Version of Record)

Published in

International Journal of Chronic Obstructive Pulmonary Disease

Publisher

Dove Medical Press

eissn

1178-2005

Acceptance date

30/09/2018

Copyright date

2018

Available date

12/08/2019

Publisher version

https://www.dovepress.com/sputum-moraxella-catarrhalis-strains-exhibit-diversity-within-and-betw-peer-reviewed-article-COPD

Language

en

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