Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.pdf (1.69 MB)
Download file

Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.

Download (1.69 MB)
journal contribution
posted on 27.11.2018, 15:24 by Pietro Roversi, Steven Johnson, Stephen G. Preston, Miles A. Nunn, Guido C. Paesen, Jonathan M. Austyn, Patricia A. Nuttall, Susan M. Lea
Two crystal structures of Japanin, an 18 kDa immune-modulatory lipocalin from the Brown Ear Tick (Rhipicephalus appendiculatus), have been determined at 2.2 and 2.4 Å resolution. In both crystal forms the protein is in complex with cholesterol, which sits in a closed pocket at the centre of the lipocalin barrel. Both crystal forms are dimers, which are also observed in solution. Molecular modelling suggests that previously-described members of a tick protein family bearing high sequence homology to Japanin are also likely to bind cholesterol or cholesterol derivatives.

Funding

SJ was funded by grant G0900888 from the UK Medical Research Council to SML; PR was funded by the Oxford Martin School Vaccine Design Institute of which SML is co-director. PR is the recipient of a Leicester LISCB-Wellcome Trust ISSF award, grant reference 204801/Z/16/Z.

History

Citation

Scientific Reports, 2017, 7:16057

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Molecular & Cell Biology

Version

VoR (Version of Record)

Published in

Scientific Reports

Publisher

Nature Research (part of Springer Nature)

eissn

2045-2322

Acceptance date

13/11/2017

Copyright date

2017

Available date

27/11/2018

Publisher version

https://www.nature.com/articles/s41598-017-16413-2

Notes

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

Language

en

Usage metrics

Categories

Keywords

Licence

Exports